Abstract: Use method of multiple internal standard method absolute concentration quantitative analysis and one component separation to improve crude oil chromatographic fingerprint technology,carry on chromatographic——mass spectrum qualitative identification on selected compound to confirm it's non-mixed compound,apply regression algorithm to set up crude oil chromatographic fingerprint mathematical model,calculate single layer crude oil combination proportion in the mixed crude oil,its result precision reach 88% comparing to the theoretical precision.Applying crude oil fingerprint technology on W1,W2 oilfield 4 commingled oil wells to carry on site separated layer productivity monitoring,it relative error is 10%~15%,and could distinguish the main oil layer accurately.The crude oil chromatograph fingerprint technology is used in commingled oil well separated layer productivity prediction,it's rapid and economical,which has the important meaning to the oil field production optimization.